Dlg2^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Dlg2^{tm1c(EUCOMM)Wtsi}
• Name: discs large MAGUK scaffold protein 2; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6449232
• Synonyms: Dlg2^flox
• Gene: Dlg2 Location: Chr7:90125880-92098455 bp, + strand Genetic Position: Chr7, 51.07 cM
• Alliance: Dlg2^{tm1c(EUCOMM)Wtsi} page
• IMPC: Dlg2 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:287325
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 91934761 of Chromosome 7 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 91936081. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:287325)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dlg2 Mutation: 59 strains or lines available

References

• Original: J:287325 Yoo T, et al., A DLG2 deficiency in mice leads to reduced sociability and increased repetitive behavior accompanied by aberrant synaptic transmission in the dorsal striatum. Mol Autism. 2020 Mar 12;11(1):19
• All: 1 reference(s)