Tchp^{tm1b(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Tchp^{tm1b(EUCOMM)Wtsi}
• Name: trichoplein, keratin filament binding; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:6449213
• Gene: Tchp Location: Chr5:114845821-114860388 bp, + strand Genetic Position: Chr5, 55.99 cM, cytoband F
• Alliance: Tchp^{tm1b(EUCOMM)Wtsi} page
• IMPC: Tchp gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:292117
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 114852212 of Chromosome 5 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 114855904. The critical exon(s) is/are thus flanked by loxP sites.A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:292117)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tchp Mutation: 21 strains or lines available

References

• Original: J:292117 Martello A, et al., Trichoplein binds PCM1 and controls endothelial cell function by regulating autophagy. EMBO Rep. 2020 Jul 3;21(7):e48192
• All: 1 reference(s)