C5ar1^{tm1.1(C5AR1)Cmac}
Targeted Allele Detail

Summary

• Symbol: C5ar1^{tm1.1(C5AR1)Cmac}
• Name: complement component 5a receptor 1; targeted mutation 1.1, Charles R Mackay
• MGI ID: MGI:6449073
• Synonyms: hC5aR1⁺
• Gene: C5ar1 Location: Chr7:15980668-15993465 bp, - strand Genetic Position: Chr7, 8.77 cM
• Alliance: C5ar1^{tm1.1(C5AR1)Cmac} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:292028
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence, Null/knockout)
• Mutations: Insertion, Intragenic deletion
• C5ar1^{tm1.1(C5AR1)Cmac} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  C5AR1 (728)

  C5ar1

  (MGI:88232)

• Mutation details: The human C5AR1 gene coding sequence (exon 2) and a loxP-flanked PGK-neomycin gene replaced the mouse gene exon 2 coding sequence. The PGK-neomycin gene was removed via cre-mediated recombination. The human gene is under the control of the endogenous mouse promoter. (J:292028)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any C5ar1 Mutation: 27 strains or lines available

References

• Original: J:292028 Lee H, et al., Human C5aR knock-in mice facilitate the production and assessment of anti-inflammatory monoclonal antibodies. Nat Biotechnol. 2006 Oct;24(10):1279-84
• All: 2 reference(s)