Pomc^tm1.1Lowl
Targeted Allele Detail

Summary

• Symbol: Pomc^tm1.1Lowl
• Name: pro-opiomelanocortin-alpha; targeted mutation 1.1, Bradford B Lowell
• MGI ID: MGI:6448946
• Synonyms: POMC-IRES-eGFP, Pomc^tm1.1Jcbr
• Gene: Pomc Location: Chr12:4004951-4010642 bp, + strand Genetic Position: Chr12, 1.99 cM
• Alliance: Pomc^tm1.1Lowl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:291152
• Parent Cell Line: W4 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (No functional change, Reporter)
• Mutation: Insertion
• Mutation details: An optimized internal ribosome entry sequence (IRES) fused to an enhanced green fluorescent protein (eGFP) sequence, followed by a FRTflanked neomycin selection cassette, was first inserted 3 bp after the Pomc stop codon within a Pomc gene. Heterozygous mice for the targeting event were crossed with mice expressing Flp-recombinase in the germline to remove neo cassette flanked by FRT sites. (J:101977, J:291152)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pomc Mutation: 24 strains or lines available

References

• Original: J:291152 Jais A, et al., PNOC(ARC) Neurons Promote Hyperphagia and Obesity upon High-Fat-Diet Feeding. Neuron. 2020 Jun 17;106(6):1009-1025.e10
• All: 2 reference(s)