Ttyh1^em1Bcgen
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ttyh1^em1Bcgen
• Name: tweety family member 1; endonuclease-mediated mutation 1, Biocytogen LLC
• MGI ID: MGI:6448353
• Gene: Ttyh1 Location: Chr7:4122418-4139206 bp, + strand Genetic Position: Chr7, 2.38 cM, cytoband A1
• Alliance: Ttyh1^em1Bcgen page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology generated a deletion of exon 4. Exon 4 deletion is predicted to result in a truncated protein of 198 amino acids thus disrupting the gene. Western blot analysis confirmed absence of TTYH1 protein but intact long non-coding RNA D030047H15Rik (NR_033548.1). qRT-PCR confirmed mRNA expression of the lncRNA. (J:291664)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ttyh1 Mutation: 42 strains or lines available

References

• Original: J:291664 Wu HN, et al., Deficiency of Ttyh1 downstream to Notch signaling results in precocious differentiation of neural stem cells. Biochem Biophys Res Commun. 2019 Jun 30;514(3):842-847
• All: 2 reference(s)