Klf5^tm2.2Jtd
Targeted Allele Detail

Summary

• Symbol: Klf5^tm2.2Jtd
• Name: Kruppel-like transcription factor 5; targeted mutation 2.2, Jin-Tang Dong
• MGI ID: MGI:6446944
• Synonyms: Klf5KR
• Gene: Klf5 Location: Chr14:99536127-99550848 bp, + strand Genetic Position: Chr14, 49.43 cM, cytoband E2.1
• Alliance: Klf5^tm2.2Jtd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:286287
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: The targeting vector is designed to insert, from 5' to 3', a loxP site, exons 2-4 of the gene, a STOP cassette, an FRT-flanked neomycin resistance cassette, and a second loxP site downstream of the endogenous exon 1 of the Klf5 gene. Endogenous exon 2 was modified to harbor a mutation at codon 358, resulting in a lysine to arginine mutation (K358R). Flp-mediated recombination removed the FRT-flanked neo cassette. Cre-mediated recombination removed the the inserted exons 2-4 and the STOP cassette allowing the expression of the K358R mutation. (J:286287)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Klf5 Mutation: 37 strains or lines available

References

• Original: J:286287 Zhang B, et al., Klf5 acetylation regulates luminal differentiation of basal progenitors in prostate development and regeneration. Nat Commun. 2020 Feb 21;11(1):997
• All: 1 reference(s)