Col1a1^{tm4(CAG-EGFP)Fcam}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm4(CAG-EGFP)Fcam}
• Name: collagen, type I, alpha 1; targeted mutation 4, Fernando Camargo
• MGI ID: MGI:6442576
• Synonyms: CARLIN, Col1a1^tm1(cCARLIN)
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm4(CAG-EGFP)Fcam} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:290327
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Lineage barcode, Reporter)
• Mutation: Insertion
• Mutation details: The targeting construct is designed as a multiplex containing 10 single guide RNAs (sgRNA) driven by separate U6 promoters inserted into the 3' UTR of an EGFP reporter driven by constitutive CAG promoter. A "barcode" 276 bp array containing target sites matching each of the expressed sgRNAs is inserted upstream of the EGFP polyadenylation site. (J:290327)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 160 strains or lines available

Notes

KH2 embryonic stem cells carrying the Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} allele, were previously re-targeted to insert an FRT-flanked PGK-Neo cassette and a promoterless hygromycin resistance cassette in the 3' UTR of the Col1a1 locus.

References

• Original: J:290327 Bowling S, et al., An Engineered CRISPR-Cas9 Mouse Line for Simultaneous Readout of Lineage Histories and Gene Expression Profiles in Single Cells. Cell. 2020 Jun 11;181(6):1410-1422.e27
• All: 2 reference(s)