Dgkg^tm1.1Yash
Targeted Allele Detail

Summary

• Symbol: Dgkg^tm1.1Yash
• Name: diacylglycerol kinase, gamma; targeted mutation 1.1, Yasuhito Shirai
• MGI ID: MGI:6441175
• Synonyms: Dgkg^tm1c
• Gene: Dgkg Location: Chr16:22287211-22475971 bp, - strand Genetic Position: Chr16, 13.37 cM
• Alliance: Dgkg^tm1.1Yash page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:288365
• Parent Cell Line: EGR-G101 (ES Cell)
• Strain of Origin: C57BL/6NSlc-Tg(CAG-EGFP,Acr-EGFP)2Osb

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: Targeting vector PRPGS00045_A_B02 was acquired from the International Knockout Mouse Consortium (IKMC). In this vector, intron 3 contains an FRT site, an IRES:lacZ trapping cassette, a loxP site, a promoter-driven neomycin resistance gene cassette, a second FRT site, and a second loxP site. Intron 5 contains a third loxP site. The lacZ and neo cassettes were removed through subsequent flp-mediated recombination, leaving a conditional-ready allele with exons 4 and 5 floxed. (J:288365)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dgkg Mutation: 59 strains or lines available

References

• Original: J:288365 Tsumagari R, et al., DGKgamma Knock-Out Mice Show Impairments in Cerebellar Motor Coordination, LTD, and the Dendritic Development of Purkinje Cells through the Activation of PKCgamma. eNeuro. 2020 Mar/Apr;7(2):ENEURO.0319-19.2020
• All: 2 reference(s)