Il6st^{tm1c(KOMP)Mbp}
Targeted Allele Detail

Summary

• Symbol: Il6st^{tm1c(KOMP)Mbp}
• Name: interleukin 6 signal transducer; targeted mutation 1c, Mouse Biology Program, UC Davis
• MGI ID: MGI:6436696
• Gene: Il6st Location: Chr13:112600604-112643394 bp, + strand Genetic Position: Chr13, 63.73 cM
• Alliance: Il6st^{tm1c(KOMP)Mbp} page
• IMPC: Il6st gene page

Mutation origin

• Mutant Cell Line: DEPD00539_7_F12
• Germline Transmission: Earliest citation of germline transmission: J:289413
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 112616156 of Chromosome 13 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 112618198. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:289413)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Il6st Mutation: 84 strains or lines available

References

• Original: J:289413 Walker EC, et al., Cortical bone maturation in mice requires SOCS3 suppression of gp130/STAT3 signalling in osteocytes. Elife. 2020 May 27;9:e56666
• All: 1 reference(s)