Mirc21^tm1.2Mrl
Targeted Allele Detail

Summary

• Symbol: Mirc21^tm1.2Mrl
• Name: microRNA cluster 21, including Mir34c through Mir34b; targeted mutation 1.2, Merck Research Laboratory
• MGI ID: MGI:6436287
• Gene: Mirc21 Location: Chr9:51014334-51014945 bp Genetic Position: Chr9, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:147112
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mirc21^tm1.2Mrl involves 2 genes/genome features (Hoatz, Btg4) View all
• Mutation details: The region containing Mir34b and Mir34c was flanked by loxP sites. The distal LoxP site was inserted into a non-conserved region approx. 400 bp downstream of Mir34c. A positive selection cassette (NeoR) was flanked by FRT recombination sites and co-inserted with the proximal LoxP site into non-conserved regions approx. 800 bp upstream of Mir34b sequence. The conditional allele was obtained after Flp-mediated removal of the selection marker. Cre-mediated recombination removed the microRNA cluster. (J:147112)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mirc21 Mutation: 5 strains or lines available

References

• Original: J:147112 Taconic, Information obtained from Taconic website. MGI Direct Data Submission. 2006-2017
• All: 1 reference(s)