Igh^tm1.1Kro
Targeted Allele Detail

Summary

• Symbol: Igh^tm1.1Kro
• Name: immunoglobulin heavy chain complex; targeted mutation 1.1, Tomohiro Kurosaki
• MGI ID: MGI:6435110
• Synonyms: B1-8^ge
• Gene: Igh Location: Chr12:113222388-115973574 bp, - strand Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:260241
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutation: Single point mutation
• Mutation details: Within the VH186.2-DFL16.1-JH2 sequence of the genomic DNA of B1-8hi mice, the Leu33 TTG codon was changed to TGG (Trp) and the B1-8 germline fragment was subcloned into a targeting vector which was electroporated into Bruce4 ES cells to generate homologous recomination. After generation of chimeric mice, the neomycin-resistance gene (neor) was removed by crossing to CAG-Flp transgenic mice. (J:260241)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igh Mutation: 47 strains or lines available

References

• Original: J:260241 Shinnakasu R, et al., Regulated selection of germinal-center cells into the memory B cell compartment. Nat Immunol. 2016 Jul;17(7):861-9
• All: 2 reference(s)