Fam13a^{tm2c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Fam13a^{tm2c(KOMP)Wtsi}
• Name: family with sequence similarity 13, member A; targeted mutation 2c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6431859
• Synonyms: Fam13a^fl
• Gene: Fam13a Location: Chr6:58910521-59001487 bp, - strand Genetic Position: Chr6, 27.93 cM
• Alliance: Fam13a^{tm2c(KOMP)Wtsi} page
• IMPC: Fam13a gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:232681
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 58960595 of Chromosome 6 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 58961360. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:232681)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fam13a Mutation: 44 strains or lines available

References

• Original: J:232681 Jin Z, et al., Regulation of nuclear-cytoplasmic shuttling and function of Family with sequence similarity 13, member A (Fam13a), by B56-containing PP2As and Akt. Mol Biol Cell. 2015 Mar 15;26(6):1160-73
• All: 2 reference(s)