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Ace2em1(ACE2)Yowa
Endonuclease-mediated Allele Detail
Nomenclature
Symbol: Ace2em1(ACE2)Yowa
Name: angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; endonuclease-mediated mutation 1, Youchun Wang
MGI ID: MGI:6431336
Synonyms: hACE2, hACE2-KI/NIFDC
Gene: Ace2  Location: ChrX:162922338-162971414 bp, + strand  Genetic Position: ChrX, 76.12 cM, cytoband F5
Mutation
origin
Strain of Origin:  C57BL/6
Mutation
description
Allele Type:    Endonuclease-mediated (Humanized sequence, Null/knockout, Reporter)
Mutation:    Insertion
 
Mutation detailsCRISPR/Cas9 technology inserted the full-length human ACE2 cDNA, IRES, and tdTomato gene into exon 2 of the mouse gene. Western blot analysis confirmed strong expression of the human gene in lung and kidney and very weak expression in spleen and liver, a similar expression patter to the mouse protein. PCR confirmed the absence of the mouse gene. (J:288243)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Disease models
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Expression
In Structures Affected by this Mutation: 3 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Ace2 Mutation:  23 strains or lines available
References
Original:  J:288243 Sun SH, A Mouse Model of SARS-CoV-2 Infection and Pathogenesis. Cell Host Microbe. 2020;20:1-10
All:  9 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
09/14/2021
MGI 6.17
The Jackson Laboratory