Gt(ROSA)26Sor^{tm1(tTA,tetO-Mtor*D2338A,-IRES-EGFP)Ebm}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(tTA,tetO-Mtor*D2338A,-IRES-EGFP)Ebm}
• Name: gene trap ROSA 26, Philippe Soriano; transgene insertion 1, Ernesto Bernal-Mizrachi
• MGI ID: MGI:6429840
• Synonyms: KD-mTOR
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(tTA,tetO-Mtor*D2338A,-IRES-EGFP)Ebm} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:252107
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inducible, Inserted expressed sequence, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(tTA,tetO-Mtor*D2338A,-IRES-EGFP)Ebm} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Mtor (MGI:1928394)	expresses a cDNA for a mouse kinase dead (D2338A) protein

• Mutation details: The transgenic construct inserted into the GT(ROSA)26Sor locus contains a splice-acceptor sequence, loxP-flanked neomycin selection cassette, a tetracycline transactivator (tTA) gene, insulator sequence, CMV promoter responsive to tTA, rabbit -globin intron, Mtor cDNA encoding a kinase dead protein, an internal ribosome entry site, and an enhanced green fluorescence protein (EGFP) cDNA. Expression of the tetracycline transactivator is turned on upon Cre-mediated recombination, and removal of the neomycin selection cassette induces Mtor expression in the absence of doxycycline. (J:252107)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 945 strains or lines available

References

• Original: J:252107 Alejandro EU, et al., Overexpression of Kinase-Dead mTOR Impairs Glucose Homeostasis by Regulating Insulin Secretion and Not beta-Cell Mass. Diabetes. 2017 Aug;66(8):2150-2162
• All: 3 reference(s)