Hprt1^{tm3(tetO-slowFT*)Sguo}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm3(tetO-slowFT*)Sguo}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 3, Shangqin Guo
• MGI ID: MGI:6423504
• Synonyms: HPRT::iH2B-FT-Slow, Hprt^{tm3(tetO/CMV-H2BC9/slowFT)Sguo}, iH2B-FT-Slow
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm3(tetO-slowFT*)Sguo} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:289588
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129S5/SvEvBrd

Mutation description

• Allele Type: Targeted (Inducible, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: The tetracycline promoter drives inducible expression along with a ubiquitous CMV of the fluorescent timer protein with the slow mutations (mCherry*M23E, E35V, K75R, L88W, A180V) fused to human H2BC9 protein for chromatin localization. (J:289588)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1273 strains or lines available

Notes

ES cell line = a mutant A17 line (A2Lox.cre) that carries a doxycycline-inducible Cre recombinase ORF flanked by loxP and lox2272 sites, upstream of a non-functional neomycin resistance (neo) gene.

References

• Original: J:289588 Eastman AE, et al., Resolving Cell Cycle Speed in One Snapshot with a Live-Cell Fluorescent Reporter. Cell Rep. 2020 Jun 23;31(12):107804
• All: 1 reference(s)