Pax6^em4Ems
Endonuclease-mediated Allele Detail

Summary

• Symbol: Pax6^em4Ems
• Name: paired box 6; endonuclease-mediated mutation 4, Elizabeth M Simpson
• MGI ID: MGI:6423491
• Synonyms: FeyCor^em4, Pax6^{Fey-em4(c.[580TG;591GA])Ems}
• Gene: Pax6 Location: Chr2:105499245-105527709 bp, + strand Genetic Position: Chr2, 55.31 cM
• Alliance: Pax6^em4Ems page

Mutation origin

• Strain of Origin: B6.Cg-Pax6^em1Ems/EmsMmmh

Mutation description

• Allele Type: Endonuclease-mediated (Epitope tag)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: CRISPR-targeting of zygotes carrying Pax6^em1Ems corrected the Pax6^Sey by introducing a T to G point mutation restoring the wild-type sequence. An additional G to A mutation was introduced further downstream. (J:287737)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pax6 Mutation: 93 strains or lines available

References

• Original: J:287737 Mirjalili Mohanna SZ, et al., Germline CRISPR/Cas9-Mediated Gene Editing Prevents Vision Loss in a Novel Mouse Model of Aniridia. Mol Ther Methods Clin Dev. 2020;Mar 14(17):478-490
• All: 1 reference(s)