Pax6^em1Ems
Endonuclease-mediated Allele Detail

Summary

• Symbol: Pax6^em1Ems
• Name: paired box 6; endonuclease-mediated mutation 1, Elizabeth M Simpson
• MGI ID: MGI:6423483
• Synonyms: Fey (FLAG-tagged Sey), Pax6^{Sey-em1(3xFLAG)Ems}
• Gene: Pax6 Location: Chr2:105499245-105527709 bp, + strand Genetic Position: Chr2, 55.31 cM
• Alliance: Pax6^em1Ems page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:287737
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: B6.Cg-Pax6^Sey/EmsMmmh

Mutation description

• Allele Type: Endonuclease-mediated (Epitope tag, Null/knockout)
• Mutations: Insertion, Single point mutation
• Mutation details: CRISPR-targeting inserted a triple FLAG tag downstream of the ATG translation start site in the Pax6^Sey allele. (J:287737)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pax6 Mutation: 92 strains or lines available

References

• Original: J:287737 Mirjalili Mohanna SZ, et al., Germline CRISPR/Cas9-Mediated Gene Editing Prevents Vision Loss in a Novel Mouse Model of Aniridia. Mol Ther Methods Clin Dev. 2020;Mar 14(17):478-490
• All: 2 reference(s)