Gt(ROSA)26Sor^{tm3(CAG-Sptlc2/Sptssa/Sptlc1,-EGFP)Rlp}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm3(CAG-Sptlc2/Sptssa/Sptlc1,-EGFP)Rlp}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 3, Richard L Proia
• MGI ID: MGI:6416541
• Synonyms: Gt(ROSA)26Sor^{tm3(CAG-SPTLC2/SPTSSA/SPTLC1,-EGFP)1Rlp}, STOP-fSPT
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm3(CAG-Sptlc2/Sptssa/Sptlc1,-EGFP)Rlp} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:285452
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: STOCK Gt(ROSA)26Sor^tm12Luo/Luo

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: Using the TARGATT integrase-mediated integration, the three individual SPT subunits (in the following order: Sptlc2, Sptssa, and Sptlc1) were cloned as a single-chain coding unit linked to EGFP by a T2A peptide sequence. A stop cassette flanked by LoxP sites was inserted between the CAG promoter and the fSPT coding unit. (J:285452)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1060 strains or lines available

Notes

This allele was produced in zygotes collected from mice carrying the Gt(ROSA)26Sor^tm12Luo allele, which was generated in (129X1/SvJ x 129S1/Sv)F1-Kitl⁺)-derived R1 ES cells on a mixed background with 129S1/SvJ, 129X1/SvJ, C57BL/6J, DBA/2J, and FVB/N.

References

• Original: J:285452 Clarke BA, et al., The Ormdl genes regulate the sphingolipid synthesis pathway to ensure proper myelination and neurologic function in mice. Elife. 2019 Dec 27;8:e51067
• All: 1 reference(s)