Ppif^em1Mure
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ppif^em1Mure
• Name: peptidylprolyl isomerase F (cyclophilin F); endonuclease-mediated mutation 1, Elizbeth Murphy
• MGI ID: MGI:6416519
• Synonyms: Ppif^p.Cys202Ser
• Gene: Ppif Location: Chr14:25694578-25700892 bp, + strand Genetic Position: Chr14, 15.06 cM
• Alliance: Ppif^em1Mure page

Mutation origin

• Strain of Origin: (C57BL/6J x CBA/J)F1

Mutation description

• Allele Type: Endonuclease-mediated (Not Specified)
• Mutation: Nucleotide substitutions
• Mutation details: The strand guide RNA (sgRNA) was designed to bind to ~20 nucleotides within the Ppif (CypD) locus and was centered near the nucleotides that predict p.Cys202Ser (C202S). Oligo2 containing four mutations (see section Genetic Alteration) was injected with the CRISPR/Cas9 components to be incorporated into the genomic DNA via homologous recombination at the cut CypD locus. Recombination of Oligo2 produced a CypD locus with the aforementioned 4 exchanges, three silent and the fourth predicting a cysteine-to-serine substitution at codon 202. (J:94077)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ppif Mutation: 18 strains or lines available

References

• Original: J:94077 Mutant Mouse Regional Resource Centers, Information obtained from the Mutant Mouse Regional Resource Centers (MMRRC). Unpublished. 2004-2015
• All: 2 reference(s)