Ect2^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ect2^{tm1c(EUCOMM)Wtsi}
• Name: ect2 oncogene; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6416160
• Synonyms: Ect2^flox
• Gene: Ect2 Location: Chr3:27151371-27207971 bp, - strand Genetic Position: Chr3, 10.82 cM, cytoband B
• Alliance: Ect2^{tm1c(EUCOMM)Wtsi} page
• IMPC: Ect2 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:287337
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_gt1 cassette was inserted at position 27190904 of Chromosome 3 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 27191800. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:287337)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ect2 Mutation: 54 strains or lines available

References

• Original: J:287337 Windmueller R, et al., Direct Comparison of Mononucleated and Binucleated Cardiomyocytes Reveals Molecular Mechanisms Underlying Distinct Proliferative Competencies. Cell Rep. 2020 Mar 3;30(9):3105-3116.e4
• All: 2 reference(s)