Anp32b^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Anp32b^{tm1c(EUCOMM)Hmgu}
• Name: acidic nuclear phosphoprotein 32 family member B; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6415694
• Synonyms: Anp32b floxed
• Gene: Anp32b Location: Chr4:46450926-46472657 bp, + strand Genetic Position: Chr4, 24.79 cM, cytoband B2
• Alliance: Anp32b^{tm1c(EUCOMM)Hmgu} page
• IMPC: Anp32b gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:275881
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_gt0 cassette was inserted at position 46468101 of Chromosome 4 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 46469020. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:275881)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Anp32b Mutation: 101 strains or lines available

References

• Original: J:275881 Chemnitz J, et al., The acidic protein rich in leucines Anp32b is an immunomodulator of inflammation in mice. Sci Rep. 2019 Mar 19;9(1):4853
• All: 2 reference(s)