Asic5^{tm2c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Asic5^{tm2c(KOMP)Wtsi}
• Name: acid-sensing ion channel family member 5; targeted mutation 2c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6415668
• Synonyms: Asic5^fl
• Gene: Asic5 Location: Chr3:81889600-81928540 bp, + strand Genetic Position: Chr3, 35.97 cM, cytoband E-F1
• Alliance: Asic5^{tm2c(KOMP)Wtsi} page
• IMPC: Asic5 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:287197
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 81911284 of Chromosome 3 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 81912249. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:287197)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Asic5 Mutation: 35 strains or lines available

References

• Original: J:287197 Kreko-Pierce T, et al., Cerebellar Ataxia Caused by Type II Unipolar Brush Cell Dysfunction in the Asic5 Knockout Mouse. Sci Rep. 2020 Feb 7;10(1):2168
• All: 1 reference(s)