Phlpp1^tm1.1Shbi
Targeted Allele Detail

Summary

• Symbol: Phlpp1^tm1.1Shbi
• Name: PH domain and leucine rich repeat protein phosphatase 1; targeted mutation 1.1, Shigeki Shimba
• MGI ID: MGI:6415195
• Synonyms: Scop^flox
• Gene: Phlpp1 Location: Chr1:106099599-106321975 bp, + strand Genetic Position: Chr1, 49.76 cM
• Alliance: Phlpp1^tm1.1Shbi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:242361
• Parent Cell Line: RENKA (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: A DNA fragment carrying a 34bp loxP sequence and Pgk-1 promoter-driven neomycin phosphotransferase gene (Pgk-neo) flanked by two Flp recognition target (frt) sites was inserted into the site 233bp upstream of exon 4. Another loxP site was introduced into the site 223bp downstream of exon 4. The neomycin selection cassette was removed via Flp-mediated recombination in the germline, leaving loxP sites that flank exon 4. (J:242361)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Phlpp1 Mutation: 53 strains or lines available

References

• Original: J:242361 Shimizu K, et al., SCOP/PHLPP1beta mediates circadian regulation of long-term recognition memory. Nat Commun. 2016 Sep 30;7:12926
• All: 2 reference(s)