Batf3^{em1.1(KOMP)Wtsi}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Batf3^{em1.1(KOMP)Wtsi}
• Name: basic leucine zipper transcription factor, ATF-like 3; endonuclease-mediated mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:6406677
• Gene: Batf3 Location: Chr1:190830611-190841140 bp, + strand Genetic Position: Chr1, 96.28 cM, cytoband H6
• Alliance: Batf3^{em1.1(KOMP)Wtsi} page
• IMPC: Batf3 gene page

Mutation origin

• Mutant Cell Line: BEPD0089_C05
• Germline Transmission: Earliest citation of germline transmission: J:82809
• Parent Cell Line: JM8A3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: Using CRISPR-targeting, the L1L2_Bact_P cassette was inserted upstream of the critical exon 2. The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 2. The critical exon 2 is thus flanked by loxP sites. Cre expression without flp expression created a reporter knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:82809)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Batf3 Mutation: 19 strains or lines available

References

• Original: J:82809 European Mouse Mutant Archive, Information obtained from the European Mouse Mutant Archive (EMMA). Unpublished. 2003-2013
• All: 1 reference(s)