Ahr^em1Bra
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ahr^em1Bra
• Name: aryl-hydrocarbon receptor; endonuclease-mediated mutation 1, Christopher A Bradfield
• MGI ID: MGI:6402393
• Synonyms: Ahr^V375A
• Gene: Ahr Location: Chr12:35547978-35584988 bp, - strand Genetic Position: Chr12, 15.78 cM
• Alliance: Ahr^em1Bra page

Mutation origin

• Strain of Origin: B6.129(FVB)-Ahr^tm3.1Bra/Bra

Mutation description

• Allele Type: Endonuclease-mediated (Not Specified)
• Mutation: Single point mutation
• Mutation details: CRISPR/Cas9-targeting in Ahr^tm3.1Bra zygotes induced a single nucleotide change. Residue 375 was changed from a valine to an alanine (p.V375A) to change the low-affinity d allele (as found in the 129 strain) to the high-affinity b allele (as found i the B6 strain). There is also a silent mutation downstream. The genetic code (with the mutation denoted by upper case) reads: gGcgatgatAtaat which was changed from gAcgatgatGtaat.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ahr Mutation: 109 strains or lines available

References

• Original: J:300133 Wilson RH, et al., Generation of an Allelic Series at the Ahr Locus Using an Edited Recombinant Approach. Toxicol Sci. 2021 Jan 22
• All: 4 reference(s)