Rhoa^tm1.1Tpal
Targeted Allele Detail

Summary

• Symbol: Rhoa^tm1.1Tpal
• Name: ras homolog family member A; targeted mutation 1.1, Teresa Palomero
• MGI ID: MGI:6401218
• Synonyms: Rhoa^co-G17V
• Gene: Rhoa Location: Chr9:108183359-108215142 bp, + strand Genetic Position: Chr9, 59.2 cM
• Alliance: Rhoa^tm1.1Tpal page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:257764
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutations: Insertion, Single point mutation
• Mutation details: Exon 3 was replaced with a minigene containing Rhoa exons 3 to 6 flanked by loxP sites followed by a neomycin selection cassette and a mutant exon 3 encoding a glycine to valine substitution at amino acid 17. The selection cassette was removed via Flp-mediated recombination. In the absence of cre recombinase, exon 2 is spliced into exons 3-6 minigene resulting in expression of a wild-type mRNA. In the presence of cre recombinase, the floxed exon 3-6 minigene is removed and the mutant G17V allele is expressed. (J:257764)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rhoa Mutation: 70 strains or lines available

References

• Original: J:257764 Cortes JR, et al., RHOA G17V Induces T Follicular Helper Cell Specification and Promotes Lymphomagenesis. Cancer Cell. 2018 Feb 12;33(2):259-273.e7
• All: 3 reference(s)