Shank3^tm1.2Bcgen
Targeted Allele Detail

Summary

• Symbol: Shank3^tm1.2Bcgen
• Name: SH3 and multiple ankyrin repeat domains 3; targeted mutation 1.2, Biocytogen LLC
• MGI ID: MGI:6399931
• Synonyms: Shank3^delta14-16
• Gene: Shank3 Location: Chr15:89383826-89444464 bp, + strand Genetic Position: Chr15, 44.96 cM
• Alliance: Shank3^tm1.2Bcgen page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:276323
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Modified isoform(s))
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 14-16 were flanked by loxP sites. In addition, an EGFP and neomycin selection cassette separated by a loxP site and flanked by FRT sites was inserted upstream of the floxed exons 14-16. Flp-mediated recombination removed the FRT-flanked EGFP and neomycin selection cassette, leaving an FRT site and floxed exons 14-16. Cre-mediated recombination deleted exons 14-16. Immunoblot analysis shows that the two main splice variants of the protein (a and c/d) are absent in several brain regions, however splice variant e is still expressed. (J:276323)

Expression

In Mice Carrying this Mutation:

2 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Shank3 Mutation: 87 strains or lines available

References

• Original: J:276323 Yoo T, et al., GABA Neuronal Deletion of Shank3 Exons 14-16 in Mice Suppresses Striatal Excitatory Synaptic Input and Induces Social and Locomotor Abnormalities. Front Cell Neurosci. 2018;12:341
• All: 4 reference(s)