Cdk4^tm3Bbd
Targeted Allele Detail

Summary

• Symbol: Cdk4^tm3Bbd
• Name: cyclin dependent kinase 4; targeted mutation 3, Mariano Barbacid
• MGI ID: MGI:6393447
• Synonyms: CDK4^K35M
• Gene: Cdk4 Location: Chr10:126899404-126903157 bp, + strand Genetic Position: Chr10, 74.5 cM, cytoband D3
• Alliance: Cdk4^tm3Bbd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:274213
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: This conditional allele was created using the FLEx switch system. A cassette containing the following elements was placed downstream of the wildtype exon 2 (the first coding exon) into intron 2: a lox511 site in forward orientation; a copy of exon 2, with a mutation changing lysine codon 35 to methionine (p.K35M), in reverse orientation; a loxP site in reverse orientation; and a lox511 site in reverse orientation. Another loxP site, in forward orientation, was inserted upstream of the wildtype exon into intron 1. Upon cre-mediated recombination, the wildtype exon will be removed and the mutated exon will be inserted in the forward orientation, taking its place, leaving a loxP site in forward orientation upstream and a lox511 site in reverse orientation downstream. The mutation in the exon renders the encoded peptide kinase-dead. (J:274213)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cdk4 Mutation: 57 strains or lines available

References

• Original: J:274213 Blasco MT, et al., Complete Regression of Advanced Pancreatic Ductal Adenocarcinomas upon Combined Inhibition of EGFR and C-RAF. Cancer Cell. 2019 Apr 15;35(4):573-587.e6
• All: 1 reference(s)