Gt(ROSA)26Sor^{tm1(CAG-Vcl/mTFP1/Venus)Hopya}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-Vcl/mTFP1/Venus)Hopya}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Sevan Hopyan
• MGI ID: MGI:6392829
• Synonyms: Gt(ROSA)Sor
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-Vcl/mTFP1/Venus)Hopya} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:275566
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change, Reporter)
• Mutation: Insertion
• Mutation details: The Ai27 targeting construct was modified to contain: CAG promoter, FRT site, loxP site, stop codons, three polyadenylation sequeces, loxP site, FRET-based full-length vinculin tension sensor (vinculin head, monomeric teal fluorescent protein1 mTFP1, spacer, Venus reporter gene and vinculin tail), WPRE, bovine growth hormone polyadenylation sequence, attB site, PGK promoter, FRT site, neomycin resistance cassette and attP site. The entire sequence was inserted between exons 1 and 2. (J:275566)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1060 strains or lines available

References

• Original: J:275566 Tao H, et al., Oscillatory cortical forces promote three dimensional cell intercalations that shape the murine mandibular arch. Nat Commun. 2019 Apr 12;10(1):1703
• All: 2 reference(s)