Sox7^{tm1b(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Sox7^{tm1b(EUCOMM)Hmgu}
• Name: SRY (sex determining region Y)-box 7; targeted mutation 1b, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6391363
• Synonyms: Sox7^lacZ
• Gene: Sox7 Location: Chr14:64181155-64188181 bp, + strand Genetic Position: Chr14, 33.34 cM, cytoband C3
• Alliance: Sox7^{tm1b(EUCOMM)Hmgu} page
• IMPC: Sox7 gene page

Mutation origin

• Mutant Cell Line: HEPD0682_2_B09
• Germline Transmission: Earliest citation of germline transmission: J:237528
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 64184750 of Chromosome 14 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 64186401. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:237528)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sox7 Mutation: 21 strains or lines available

References

• Original: J:237528 Lilly AJ, et al., Interplay between SOX7 and RUNX1 regulates hemogenic endothelial fate in the yolk sac. Development. 2016 Dec 01;143(23):4341-4351
• All: 2 reference(s)