Gmnc^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Gmnc^{tm1c(KOMP)Wtsi}
• Name: geminin coiled-coil domain containing; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6391362
• Synonyms: Gmnc^Fl
• Gene: Gmnc Location: Chr16:26775985-26810424 bp, - strand Genetic Position: Chr16, 18.56 cM
• Alliance: Gmnc^{tm1c(KOMP)Wtsi} page
• IMPC: Gmnc gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:284599
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 26781378 of Chromosome 16 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 26783210. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:284599)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gmnc Mutation: 26 strains or lines available

References

• Original: J:284599 Lalioti ME, et al., GemC1 is a critical switch for neural stem cell generation in the postnatal brain. Glia. 2019 Dec;67(12):2360-2373
• All: 1 reference(s)