Acot13^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Acot13^{tm1c(EUCOMM)Wtsi}
• Name: acyl-CoA thioesterase 13; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6390977
• Synonyms: Them2^Flox
• Gene: Acot13 Location: Chr13:25001938-25015472 bp, - strand Genetic Position: Chr13, 10.69 cM, cytoband A3.2
• Alliance: Acot13^{tm1c(EUCOMM)Wtsi} page
• IMPC: Acot13 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:280248
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_gt0 cassette was inserted at position 25004349 of Chromosome 13 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 25005230. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:280248)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Acot13 Mutation: 11 strains or lines available

References

• Original: J:280248 Alves-Bezerra M, et al., Thioesterase Superfamily Member 2 Promotes Hepatic VLDL Secretion by Channeling Fatty Acids Into Triglyceride Biosynthesis. Hepatology. 2019 Aug;70(2):496-510
• All: 1 reference(s)