Ube2t^{tm1d(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Ube2t^{tm1d(EUCOMM)Hmgu}
• Name: ubiquitin-conjugating enzyme E2T; targeted mutation 1d, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6390895
• Gene: Ube2t Location: Chr1:134890303-134901900 bp, + strand Genetic Position: Chr1, 58.24 cM
• Alliance: Ube2t^{tm1d(EUCOMM)Hmgu} page
• IMPC: Ube2t gene page

Mutation origin

• Mutant Cell Line: HEPD0723_7_A07
• Germline Transmission: Earliest citation of germline transmission: J:157065
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: he L1L2_Bact_P cassette was inserted at position 134968759 of Chromosome 1 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 134969570. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression resulted in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ube2t Mutation: 31 strains or lines available

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 1 reference(s)