Opn5^{tm1a(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Opn5^{tm1a(KOMP)Wtsi}
• Name: opsin 5; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:6387219
• Gene: Opn5 Location: Chr17:42867674-42922286 bp, - strand Genetic Position: Chr17, 19.65 cM, cytoband B3
• Alliance: Opn5^{tm1a(KOMP)Wtsi} page
• IMPC: Opn5 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:279052
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s). The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. (J:279052)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Opn5 Mutation: 22 strains or lines available

References

• Original: J:279052 Nguyen MT, et al., An opsin 5-dopamine pathway mediates light-dependent vascular development in the eye. Nat Cell Biol. 2019 Apr;21(4):420-429
• All: 2 reference(s)