Gdpd1^em1Osb
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gdpd1^em1Osb
• Name: glycerophosphodiester phosphodiesterase domain containing 1; endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University
• MGI ID: MGI:6387028
• Synonyms: Gdpd1^-20
• Gene: Gdpd1 Location: Chr11:86924693-86964888 bp, - strand Genetic Position: Chr11, 51.87 cM, cytoband C
• Alliance: Gdpd1^em1Osb page

Mutation origin

• Strain of Origin: (C57BL/6NJcl x DBA/2NJcl)F2

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology generated a 20 bp deletion (5-TCGTCCACCGCGGCTTTTTG-3) in the first exon. The sgRNA sequence used for microinjection was 5'-GAGAAGGCAAAAAGCCGCGG-3' for the first exon. The deletion causes a frameshift mutation leading to a premature termination codon after the 17th amino acid. (J:279308)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Gdpd1 Mutation: 17 strains or lines available

References

• Original: J:279308 Fujihara Y, et al., Identification of multiple male reproductive tract-specific proteins that regulate sperm migration through the oviduct in mice. Proc Natl Acad Sci U S A. 2019 Sep 10;116(37):18498-18506
• All: 1 reference(s)