Tg(Chat-flpo)22Majh
Transgene Detail

Summary

• Symbol: Tg(Chat-flpo)22Majh
• Name: transgene insertion 22, Marthe Howard
• MGI ID: MGI:6387001
• Synonyms: ChAT-FlpO
• Transgene: Tg(Chat-flpo)22Majh Location: unknown
• Alliance: Tg(Chat-flpo)22Majh page

Transgene origin

• Strain of Origin: C57BL/6 x SJL

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Chat-flpo)22Majh expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Chat (MGI:88392)

• Mutation details: The 176 kb bacterial artificial chromosome, RP24-256F2, containing the entire Chat and Slc18a3 (VAChT) genes was modified to insert an optimized FLP recombinase (FLPo) followed by a roxP-flanked PGK-prokaryotic gb2 promoter-neoR/kanR cassette under the direction of the Chat promoter. Induction of Dre recombinase removes the kanamycin cassette in recombineering competent bacteria. Next, a 3X Stop is inserted into the Slc18a3 (or VAChT) blocking transcription. (J:101977)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Chat (mouse) Summary of all recombinase alleles driven by Chat. Alliance mouse Chat gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 1 reference(s)