Tg(CAG-cas9,Rnu6-gRNA:Trp53,Krt14-a)E3BYtchn
Transgene Detail

Summary

• Symbol: Tg(CAG-cas9,Rnu6-gRNA:Trp53,Krt14-a)E3BYtchn
• Name: transgene insertion E3B, You-Tzung Chen
• MGI ID: MGI:6386746
• Synonyms: CAGLSL-Cas9, ICE CRIM
• Transgene: Tg(CAG-cas9,Rnu6-gRNA:Trp53,Krt14-a)E3BYtchn Location: unknown
• Alliance: Tg(CAG-cas9,Rnu6-gRNA:Trp53,Krt14-a)E3BYtchn page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Endonuclease, Reporter)
• Mutation: Insertion
• Mutation details: Three transgenes were co-injected expressing cas9 under the conrol of the ubiquitous CAG promoter, Rnu6 promoter driving expression of a guide RNA targeting Trp52 and agouti allele under the control of the Krt14 promoter. The E3B line was generated. (J:283235)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:283235 Fan HH, et al., P53 ICE CRIM mouse: a tool to generate mutant allelic series in somatic cells and germ lines for cancer studies. FASEB J. 2019 Apr;33(4):5571-5584
• All: 1 reference(s)