Slc18a2^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Slc18a2^{tm1c(EUCOMM)Wtsi}
• Name: solute carrier family 18 (vesicular monoamine), member 2; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6386316
• Gene: Slc18a2 Location: Chr19:59249328-59284444 bp, + strand Genetic Position: Chr19, 54.64 cM
• Alliance: Slc18a2^{tm1c(EUCOMM)Wtsi} page
• IMPC: Slc18a2 gene page

Mutation origin

• Mutant Cell Line: EPD0242_2_F06
• Germline Transmission: Earliest citation of germline transmission: J:295948
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Pgk_P
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 59250939 of Chromosome 19 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 59251955. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:295948)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc18a2 Mutation: 29 strains or lines available

References

• Original: J:295948 Sakano D, et al., VMAT2 Safeguards Beta-Cells Against Dopamine Cytotoxicity Under High-Fat Diet Induced Stress. Diabetes. 2020 Aug 21;69(11):2377-2391
• All: 1 reference(s)