Trf^tm1Refl
Targeted Allele Detail

Summary

• Symbol: Trf^tm1Refl
• Name: transferrin; targeted mutation 1, Robert E Fleming
• MGI ID: MGI:6385876
• Synonyms: Tf^N-bl
• Gene: Trf Location: Chr9:103086075-103107485 bp, - strand Genetic Position: Chr9, 55.03 cM, cytoband F1-F3
• Alliance: Trf^tm1Refl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:282105
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Two A to T nucleotide substitutions were introduced resulting in a tyrosine to phenylalanine change at amino acid 114 (Y114F) and at amino acid 207 (Y207F). A FRT-flanked neomycin resistance cassette was inserted downstream of the two mutations. These two mutations abrogate iron binding in the N lobe of transferrin. (J:282105)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Trf Mutation: 42 strains or lines available

References

• Original: J:282105 Parrow NL, et al., Lobe specificity of iron binding to transferrin modulates murine erythropoiesis and iron homeostasis. Blood. 2019 Oct 24;134(17):1373-1384
• All: 1 reference(s)