Gt(ROSA)26Sor^{tm4.1(CAG-HRP)Ddg}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm4.1(CAG-HRP)Ddg}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 4.1, David D Ginty
• MGI ID: MGI:6385515
• Synonyms: ROSA26^LSL-SV-HRP
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm4.1(CAG-HRP)Ddg} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The targeting vector is created by replacing the tdTomato cassette in Gt(ROSA)26Sor^{tm65.1(CAG-tdTomato)Hze} with DR-SV-HRP, a dual (Cre and Flp) recombinase (DR)-dependent, synaptic vesicle (SV)-targeted, horse radish peroxidase (HRP) reporter cassette. The following was introduced to intron 1 of the mouse Gt(ROSA)26Sor gene - CAG-FRT-Stop-FRT-loxP-Stop-loxP-SV (synaptophysin protein, a synaptic vesicle targeting peptide)-HRP (horse radish peroxidase)-WPRE (Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element)-bGHpA (bovine growth hormone polyadenylation signal)-attB-PGK-NeoR-PGKpA-attP. Flp-mediated recombination removed the FRT-flanked stop cassette. (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 944 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 1 reference(s)