H13^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: H13^{tm1c(KOMP)Wtsi}
• Name: histocompatibility 13; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6384976
• Synonyms: SPP^flox
• Gene: H13 Location: Chr2:152511381-152550590 bp, + strand Genetic Position: Chr2, 75.41 cM
• Alliance: H13^{tm1c(KOMP)Wtsi} page

Mutation origin

• Mutant Cell Line: EPD0867_5_D04
• Germline Transmission: Earliest citation of germline transmission: J:282788
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 152522482 of Chromosome 2 upstream of exon 3(Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 3 at position 152523314. Flp-mediated recombination removed the FRT-flanked neo cassette leaving exon 3 floxed. (J:282788)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any H13 Mutation: 39 strains or lines available

References

• Original: J:282788 Wang S, et al., Absence of Signal Peptide Peptidase, an Essential Herpes Simplex Virus 1 Glycoprotein K Binding Partner, Reduces Virus Infectivity In Vivo. J Virol. 2019 Dec 1;93(23)
• All: 2 reference(s)