Mir290a^{em2.1(Snrpn-EGFP)Jae}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Mir290a^{em2.1(Snrpn-EGFP)Jae}
• Name: microRNA 290a; endonuclease-mediated mutation 2.1, Rudolf Jaenisch
• MGI ID: MGI:6383986
• Synonyms: MIR290--CAST^SE-RGM-eGFP, MIR290-SE-G
• Gene: Mir290a Location: Chr7:3267300-3267382 bp, + strand Genetic Position: Chr7, 1.88 cM
• Alliance: Mir290a^{em2.1(Snrpn-EGFP)Jae} page

Mutation origin

• Strain of Origin: CAST/Ei

Mutation description

• Allele Type: Endonuclease-mediated (Reporter)
• Mutation: Insertion
• Mutation details: CRISPR/cas9 endonuclease-mediated genome editing is used to target the super enhancer (SE) sequences ~15 kb proximal of the microRNA 290a locus (Mir290a) on chromosome 7, as well as Snrpn::eGFP::WPRE::BHGpA::loxP-PGK-Puro-loxP sequences (encoding the minimal Snrpn promoter [including conserved elements between human and mouse and containing the endogenous imprinted DMR region], the enhanced green fluorescent protein, a WPRE sequence, a BGH polyadenylation sequence and a loxP-flanked PGK-Puro selection cassette). Junction PCR-SNP analyses identified ES cells with the Snrpn::eGFP::WPRE::BHGpA sequences inserted into the Mir290a SE on the CAST chromosome. Those ES cells were transfected with a Cre recombinase-expressing plasmid to remove the PGK-Puro cassette. (J:282716)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mir290a Mutation: 5 strains or lines available

References

• Original: J:282716 Song Y, et al., Dynamic Enhancer DNA Methylation as Basis for Transcriptional and Cellular Heterogeneity of ESCs. Mol Cell. 2019 Sep 5;75(5):905-920.e6
• All: 1 reference(s)