Esr1^tm1Neg
Targeted Allele Detail

Summary

• Symbol: Esr1^tm1Neg
• Name: estrogen receptor 1 (alpha); targeted mutation 1, Masahiko Negishi
• MGI ID: MGI:6383863
• Synonyms: Esr1 S216A
• Gene: Esr1 Location: Chr10:4561989-4955633 bp, + strand Genetic Position: Chr10, 2.03 cM
• Alliance: Esr1^tm1Neg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:300412
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: A targeting vector containing an ACN self-excising loxP-flanked neomycin cassette and a diptheria toxin (DTA) gene is used to replace the serine residue at position 216 of exon 3 with an alanine residue. The left homology arm included all of exons 2, 3, and 4 (including the S216A substitution codon), while the right homology arm contained part of intron 4. (J:300412)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Esr1 Mutation: 67 strains or lines available

References

• Original: J:300412 Shindo S, et al., Estrogen receptor alpha phosphorylated at Ser216 confers inflammatory function to mouse microglia. Cell Commun Signal. 2020 Jul 29;18(1):117
• All: 2 reference(s)