Zcchc8^em1Arman
Endonuclease-mediated Allele Detail

Summary

• Symbol: Zcchc8^em1Arman
• Name: zinc finger, CCHC domain containing 8; endonuclease-mediated mutation 1, Mary Armanios
• MGI ID: MGI:6378994
• Synonyms: Zcchc8^-
• Gene: Zcchc8 Location: Chr5:123836365-123859107 bp, - strand Genetic Position: Chr5, 63.14 cM, cytoband F
• Alliance: Zcchc8^em1Arman page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Insertion
• Mutation details: First, a single guide RNA (5'-TGAACATTCTGACAAGACCC-3') was designed to target sequences in exon 2. Next, a DNA oligo for homology directed repair (HDR) was designed to target a 33 bp insertion (BamHI site, Flag tag sequence and STOP sequence) into exon 2 that introduced a premature stop codon at amino acid 93 (P83delinsGSDYKDDDDK*). (J:281519)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Zcchc8 Mutation: 27 strains or lines available

References

• Original: J:281519 Gable DL, et al., ZCCHC8, the nuclear exosome targeting component, is mutated in familial pulmonary fibrosis and is required for telomerase RNA maturation. Genes Dev. 2019 Oct 1;33(19-20):1381-1396
• All: 1 reference(s)