Runx1^tm5Toku
Targeted Allele Detail

Summary

• Symbol: Runx1^tm5Toku
• Name: runt related transcription factor 1; targeted mutation 5, Tsukasa Okuda
• MGI ID: MGI:6378825
• Synonyms: Runx1^KTAMK
• Gene: Runx1 Location: Chr16:92398354-92622962 bp, - strand Genetic Position: Chr16, 53.7 cM
• Alliance: Runx1^tm5Toku page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:281491
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutation: Nucleotide substitutions
• Mutation details: Using site-directed mutagenesis, arginine codons 206 and 210 in exon 4 were changed to lysine codons (p.R206K, p.R210K). A puromycin resistance gene cassette was inserted downstream of the targeted codons in exon 4. The two targeted arginines are normally methylated as part of the regulation of the peptide's activity; lysines cannot be methylated. (J:281491)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Runx1 Mutation: 34 strains or lines available

Notes

E14-strain ES cells

References

• Original: J:281491 Mizutani S, et al., Loss of RUNX1/AML1 arginine-methylation impairs peripheral T cell homeostasis. Br J Haematol. 2015 Sep;170(6):859-73
• All: 1 reference(s)