Ensatm1c(EUCOMM)Hmgu
Targeted Allele Detail
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Symbol: |
Ensatm1c(EUCOMM)Hmgu |
Name: |
endosulfine alpha; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH |
MGI ID: |
MGI:6377562 |
Synonyms: |
EnsaloxP |
Gene: |
Ensa Location: Chr3:95532304-95539413 bp, + strand Genetic Position: Chr3, 40.74 cM
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Alliance: |
Ensatm1c(EUCOMM)Hmgu page
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IMPC: |
Ensa gene page |
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Mutant Cell Line: |
HEPD0901_1_A07 |
Germline Transmission: |
Earliest citation of germline transmission:
J:271162
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Parent Cell Line: |
JM8.N4 (ES Cell)
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Strain of Origin: |
C57BL/6N
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Project Collection: |
EUCOMM
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Allele Type: |
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Targeted (Conditional ready, No functional change) |
Mutation: |
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Insertion
Vector: L1L2_GT0_LF2A_LacZ_BetactP_neo
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Mutation details: The L1L2_GT0_LF2A_LacZ_BetactP_neo cassette was inserted at position 95533606 of Chromosome 3 upstream of the critical exons 2 and 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons 2 and 3 at position 95536162. The critical exons 2 and 3 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml
(J:271162)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Ensa Mutation: |
30 strains or lines available
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Original: |
J:271162 Hached K, et al., ENSA and ARPP19 differentially control cell cycle progression and development. J Cell Biol. 2019 Feb 4;218(2):541-558 |
All: |
1 reference(s) |
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