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Ensatm1c(EUCOMM)Hmgu
Targeted Allele Detail
Summary
Symbol: Ensatm1c(EUCOMM)Hmgu
Name: endosulfine alpha; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
MGI ID: MGI:6377562
Synonyms: EnsaloxP
Gene: Ensa  Location: Chr3:95532304-95539413 bp, + strand  Genetic Position: Chr3, 40.74 cM
Alliance: Ensatm1c(EUCOMM)Hmgu page
IMPC: Ensa gene page
Mutation
origin
Mutant Cell Line:  HEPD0901_1_A07
Germline Transmission:  Earliest citation of germline transmission: J:271162
Parent Cell Line:  JM8.N4 (ES Cell)
Strain of Origin:  C57BL/6N
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Conditional ready, No functional change)
Mutation:    Insertion     Vector: L1L2_GT0_LF2A_LacZ_BetactP_neo
 
Mutation detailsThe L1L2_GT0_LF2A_LacZ_BetactP_neo cassette was inserted at position 95533606 of Chromosome 3 upstream of the critical exons 2 and 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons 2 and 3 at position 95536162. The critical exons 2 and 3 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:271162)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Ensa Mutation:  30 strains or lines available
References
Original:  J:271162 Hached K, et al., ENSA and ARPP19 differentially control cell cycle progression and development. J Cell Biol. 2019 Feb 4;218(2):541-558
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/22/2024
MGI 6.24
The Jackson Laboratory