Atp2c1^{tm1b(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Atp2c1^{tm1b(KOMP)Wtsi}
• Name: ATPase, Ca++-sequestering; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:6369819
• Synonyms: Spca1^KO
• Gene: Atp2c1 Location: Chr9:105288561-105398456 bp, - strand Genetic Position: Chr9, 56.61 cM, cytoband F1
• Alliance: Atp2c1^{tm1b(KOMP)Wtsi} page

Mutation origin

• Mutant Cell Line: EPD0858_4_C07
• Germline Transmission: Earliest citation of germline transmission: J:266546
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 105328730 of Chromosome 9 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 105330469. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:266546)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Atp2c1 Mutation: 76 strains or lines available

References

• Original: J:266546 Brown JM, et al., Secretory pathway calcium ATPase 1 (SPCA1) controls mouse neural tube closure by regulating cytoskeletal dynamics. Development. 2018 Oct 5;145(19):dev170019
• All: 1 reference(s)