Mib2^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Mib2^{tm1d(EUCOMM)Wtsi}
• Name: mindbomb E3 ubiquitin protein ligase 2; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:6369362
• Synonyms: Mib2^-
• Gene: Mib2 Location: Chr4:155739134-155753655 bp, - strand Genetic Position: Chr4, 86.86 cM
• Alliance: Mib2^{tm1d(EUCOMM)Wtsi} page
• IMPC: Mib2 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:280242
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_gt2 cassette was inserted at position 155742841 of Chromosome 4 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 155744978. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:280242)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mib2 Mutation: 59 strains or lines available

References

• Original: J:280242 Uematsu A, et al., The E3 ubiquitin ligase MIB2 enhances inflammation by degrading the deubiquitinating enzyme CYLD. J Biol Chem. 2019 Sep 20;294(38):14135-14148
• All: 1 reference(s)