Mib2^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Mib2^{tm1c(EUCOMM)Wtsi}
• Name: mindbomb E3 ubiquitin protein ligase 2; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6369361
• Synonyms: Mib2 flox
• Gene: Mib2 Location: Chr4:155739134-155753655 bp, - strand Genetic Position: Chr4, 86.86 cM
• Alliance: Mib2^{tm1c(EUCOMM)Wtsi} page
• IMPC: Mib2 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:280242
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_gt2 cassette was inserted at position 155742841 of Chromosome 4 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 155744978. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:280242)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mib2 Mutation: 59 strains or lines available

References

• Original: J:280242 Uematsu A, et al., The E3 ubiquitin ligase MIB2 enhances inflammation by degrading the deubiquitinating enzyme CYLD. J Biol Chem. 2019 Sep 20;294(38):14135-14148
• All: 2 reference(s)