Stk33^tm1.1Csll
Targeted Allele Detail

Summary

• Symbol: Stk33^tm1.1Csll
• Name: serine/threonine kinase 33; targeted mutation 1.1, Claudia Scholl
• MGI ID: MGI:6368782
• Synonyms: Stk33.lox
• Gene: Stk33 Location: Chr7:108878430-109038288 bp, - strand Genetic Position: Chr7, 57.21 cM
• Alliance: Stk33^tm1.1Csll page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:253666
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to replace the Stk33 locus between exon 6 and exon 8 with a cassette containing exon 7 of Stk33, a neomycin resistance gene flanked by FRT sites, and loxP sites surrounding exon 7 and the neomycin cassette. The neomycin cassette was removed through Flp-mediated recombination via crossing to ACTB:FLPe B6J mice to obtain the conditional strain that contains loxP recombination sites flanking exon 7 of Stk33. (J:253666)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Stk33 Mutation: 29 strains or lines available

References

• Original: J:253666 Martins LR, et al., Stk33 is required for spermatid differentiation and male fertility in mice. Dev Biol. 2018 Jan 1;433(1):84-93
• All: 1 reference(s)